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Updated: Mar 7, 2023

Porous layer open tubular (PLOT) columns are traditionally built with particles that are adhered to the tubing walls. These columns have unique selectivity and provide a great alternative when gaseous samples need to be separated, but these columns also have been used to separate higher boiling point analytes.

There are many different commercially available stationary phases of PLOT columns, including alumina-based columns, molecular sieves, and porous polymers. Alumina-based columns have an aluminium oxide stationary phase that is then deactivated with different salts.

These columns have high capacity, superior loading ability, and produce symmetrical peaks. Molecular sieve columns are designed specifically for permanent gas separations because the columns have high retention. Porous polymer columns are highly hydrophobic, making them more applicable to analyzing a wider range of samples.

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